Generator

Part:BBa_K1472614:Design

Designed by: Lau Tsz Kit   Group: iGEM14_CityU_HK   (2014-11-01)


∆9, ∆12, ∆15 desaturases with LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1278
    Illegal BamHI site found at 673
    Illegal BamHI site found at 1638
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 497
    Illegal AgeI site found at 1459
    Illegal AgeI site found at 1723
    Illegal AgeI site found at 1861
    Illegal AgeI site found at 2694
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The desaturase genes in plasmids were introduced into E. coli for amplification. The final construct includes iGEM provided RBS (BBa_B0034).


Source

All the desaturase sequences are derived from the Synechocystis sp. but optimised for the expression in E. coli.

References